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Participants V
Status Dormant
Niche Electronics
Purpose World domination

Interface (older) UV/Vis detectors with a nanode instead of a chartrecorder, write interpretation software

Got a couple of different Uv/VIS detectors 2 are from the 80's and 1 is built more recently (>1995). One is a Varian that unfortunately exceeded its lamp burning hours and will most likely not bee operating until i get a new lamp.

The 2 operational ones are an Alltech 450 fixed 254 nm UV spectrometer which is designed to function as a preperative HPLC detector but should work fine as a normal UV HPLC detector. The other one is older but actually nicer since it has a dial to adjust the wavelenght.

The 2 working detectors have integrator ports that read 1V at 1AU (which means 90% of all photons are absorbed)

The plan is to connect these detectors to the a/d converter of the nanode, write a program for the nanode that sets set the analogReference to INTERNAL, makes it read the analog port at a defined interval and printout the 10bit value including a timestamp. Write software that reads the data provided by the nanode to interpretate the signals over a defined timeline (HPLC run time) and provide provide a textual and graphical presentation of the data acquired.

Dreamer opted me to take a look and consider PySide for writing the GUI, thus i decided to take a look at PyQt and learned that PyQt is an amazing framework :)


1. Solder nanode kit together - Done (after sitting quietly in a corner soldering half a sunday :P).

2. Measure the peak voltage at full absorbance on both spectrometers - Done

It seems that the detectors i am working with provide around 1V, but i did measure negative current. I am not planning to use the reference port for ranging my voltage but i'm not sure how well this nanode can handle an accidental low negative voltage.

3, Write a simple program for the nanode to do the writing. - Wrote a simple program for this and will have to improve/add features now that the GUI is somewhat working.

4. Write a simple GUI that will draw the absorbance peaks niceley over a timeframe using PyQt. - Done

[1] http://arduino.cc/en/Reference/AnalogReference