Difference between revisions of "UV/Vino"

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{{Project
 
{{Project
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|ProjectStatus=Active
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|picture=Uvvino.png
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|ProjectStatus=Dormant
 
|ProjectNiche=Electronics
 
|ProjectNiche=Electronics
 
|ProjectPurpose=World domination
 
|ProjectPurpose=World domination
 
}}
 
}}
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Interface older style UV/Vis detectors with a nanode instead of a chartrecorder, write interpretation software   
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Interface (older) UV/Vis detectors with a nanode instead of a chartrecorder, write interpretation software   
  
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Got a couple of different Uv/VIS detectors from the mid 80's (2 Varian's, 1 is not really operational since the deuterium lamp is very weak and then there is a nice straighforward really oldskool one but i cannot remember the brand) they are normally hooked up to a chart recorder the varian ones seem to send a 0-12V signal and the other one a 0-1v signal corresponding to the Au (absorbance). The plan is to connect these detectors with the a/d converter of the nanode, write a program for the nanode that makes it read the analog port at a defined interval and then print the 10bit value including a timestamp and write software that reads the data provided by the nanode to interpretate the signals over a defined timeline (HPLC run time) providing a textual and graphical presentation.
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Got a couple of different Uv/VIS detectors 2 are from the 80's and 1 is built more recently (>1995). One is a Varian that unfortunately exceeded its lamp burning hours and will most likely not bee operating until i get a new lamp.  
  
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For the lulz i started to play around with SDL libraries to draw the peaks but this does not feel very sane and im switching to PyGTK, a great oportunity to get to learn a bit more on this (and of course feel free to bash my code once it is done because it _will_ bee ugly)
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The 2 operational ones are an Alltech 450 fixed 254 nm UV spectrometer which is designed to function as a preperative HPLC detector but should work fine as a normal UV HPLC detector. The other one is older but actually nicer since it has a dial to adjust the wavelenght.
  
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planning:
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The 2 working detectors have integrator ports that read 1V at 1AU (which means 90% of all photons are absorbed)
  
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1. Solder nanode kit together - done (after sitting quietly in a corner soldering half a sunday :P).
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The plan is to connect these detectors to the a/d converter of the nanode, write a program for the nanode that sets set the analogReference to INTERNAL, makes it read the analog port at a defined interval and printout the 10bit value including a timestamp. Write software that reads the data provided by the nanode to interpretate the signals over a defined timeline (HPLC run time) and provide provide a textual and graphical presentation of the data acquired.
  
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2. Measure the peak voltage at full absorbance on both spectrometers, the varian should give 12v at full absorbance and thus needs to bee downregulated to a bit under 5V. Knowing nothing about electronics i learned atleast that a series of resistors is a bad idea due to non constant load therefore i hope a simple voltage regulator will suffice but if anyone has input on this i would love to hear about it. The other detector is supposed to give 1v at full absorbance  If correct then the 1 volt can hopefully bee ranged.  - About to check this out (06-03-2013)
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Dreamer opted me to take a look and consider PySide for writing the GUI, thus i decided to take a look at PyQt and learned that PyQt is an amazing framework :)
  
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3, Write a simple program for the nanode to do the writing. - Wrote a simple program for this and will improve/add features once the GUI is somewhat together
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Planning:
  
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4. Write a simple GUI that will draw the absorbance peaks niceley over a timeframe using PyGTK. - Reading tutorials :)
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1. Solder nanode kit together - Done (after sitting quietly in a corner soldering half a sunday :P).
 +
 
 +
2. Measure the peak voltage at full absorbance on both spectrometers - Done
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It seems that the detectors i am working with provide around 1V, but i did measure negative current. I am not planning to use the reference port for ranging my voltage but i'm not sure how well this nanode can handle an accidental low negative voltage.
 +
 
 +
3, Write a simple program for the nanode to do the writing. - Wrote a simple program for this and will have to improve/add features now that the GUI is somewhat working.
 +
 
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4. Write a simple GUI that will draw the absorbance peaks niceley over a timeframe using PyQt. - Done
 +
 
 +
[1] http://arduino.cc/en/Reference/AnalogReference

Latest revision as of 02:07, 15 February 2017

Projects
Uvvino.png
Participants V
Skills
Status Dormant
Niche Electronics
Purpose World domination

Interface (older) UV/Vis detectors with a nanode instead of a chartrecorder, write interpretation software

Got a couple of different Uv/VIS detectors 2 are from the 80's and 1 is built more recently (>1995). One is a Varian that unfortunately exceeded its lamp burning hours and will most likely not bee operating until i get a new lamp.

The 2 operational ones are an Alltech 450 fixed 254 nm UV spectrometer which is designed to function as a preperative HPLC detector but should work fine as a normal UV HPLC detector. The other one is older but actually nicer since it has a dial to adjust the wavelenght.

The 2 working detectors have integrator ports that read 1V at 1AU (which means 90% of all photons are absorbed)

The plan is to connect these detectors to the a/d converter of the nanode, write a program for the nanode that sets set the analogReference to INTERNAL, makes it read the analog port at a defined interval and printout the 10bit value including a timestamp. Write software that reads the data provided by the nanode to interpretate the signals over a defined timeline (HPLC run time) and provide provide a textual and graphical presentation of the data acquired.

Dreamer opted me to take a look and consider PySide for writing the GUI, thus i decided to take a look at PyQt and learned that PyQt is an amazing framework :)

Planning:

1. Solder nanode kit together - Done (after sitting quietly in a corner soldering half a sunday :P).

2. Measure the peak voltage at full absorbance on both spectrometers - Done

It seems that the detectors i am working with provide around 1V, but i did measure negative current. I am not planning to use the reference port for ranging my voltage but i'm not sure how well this nanode can handle an accidental low negative voltage.

3, Write a simple program for the nanode to do the writing. - Wrote a simple program for this and will have to improve/add features now that the GUI is somewhat working.

4. Write a simple GUI that will draw the absorbance peaks niceley over a timeframe using PyQt. - Done

[1] http://arduino.cc/en/Reference/AnalogReference

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